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. 2016 Feb 3;7:61. doi: 10.3389/fmicb.2016.00061

Figure 3.

Figure 3

Crystal structure of FS5929R1 PR. (A) Ribbon diagram of the PR, which forms a homodimer. The α-helices and β-strands are colored in red and yellow, respectively. The flap regions (residues 43–58) are highlighted in orange. (B) Locations of mutated residues on the PR. The red, yellow, and green spheres indicate the locations of the major DRV resistance, minor DRV resistance, and other mutations, respectively, that appeared after the DRV-resistance induction experiment using FS5929. Mutations that originally existed in FS5929 are shown in gray spheres. (C) Highlighted structure around the flaps in the PR. The mutations are shown as sticks. The mutations V32I, I47V, and I50V, which appeared during the in vitro selection, are highlighted with red letters. (D) Superposition of our crystal structures of the PRs (gray) with the crystal structures of the open form [PDB IDs: 2PC0 (cyan), 1TW7 (purple), 4NPU (orange), and 3UF3 (navy)]. (E) Superposition of our crystal structures of the PRs with a crystal structure of the semi-open form [PDB IDs: 1HHP (green)].