Table 2.
List of PR mutations detected during in vitro selection.
| N* | DRV resistance mutations | Other† | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Major† | Minor† | ||||||||||||
| 47 | 50 | 76 | 11 | 32 | 33 | 89 | 54 | 60 | 62 | 82 | |||
| NL4-3 | I | I | L | V | V | L | L | I | D | I | V | ||
| FS5929 | (Day 0)‡ | – | – | – | – | – | F | – | V | – | V | F | |
| FS5929R | (Day 154)‡ | 4$ | V | V | – | I | I | F | V | V | E | – | F |
| 3 | V | V | – | – | I | F | V | V | E | – | F | ||
| 2 | V | V | – | I | I | F | V | V | E | – | L | ||
| 1 | V | – | V | I | I | F | V | V | E | – | F | ||
| 1 | V | – | V | – | I | F | V | V | E | – | F | ||
| 1 | V | – | – | I | I | F | V | A | E | – | F | ||
| 1 | V | – | – | I | I | F | V | V | E | – | F | ||
*
The number of clones with the combination of mutations.
†
Classification of the IAS-USA mutation list at June/July 2014.
‡
FS5929 and FS5929R denote the input virus for the in vitro selection (day 0) and the virus mixture obtained at the day 154 in this study.
The sequence contains mutations: L10I, L23I, L33F, M46I, F53L, I54V, G57R, Q58E, I62V, L63P, H69R, A71V, G73S, V77I, V82F, L90M, and I93L, compared with NL4-3. L33F is reported to be associated with DRV-resistance mutations.
$
The major virus in the virus mixture obtained at the day 154 (FS5929R) was defined as FS5929R1.