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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Cancer Genet. 2015 Dec 15;209(0):42–49. doi: 10.1016/j.cancergen.2015.12.004

Figure 2.

Figure 2

Example case (sample 16) showing a high grade pancreatic intraductal neoplasm in a patient with concurrent invasive pancreatic adenocarcinoma. The estimated neoplastic cellularity was 20% - 30% for the area circled with a slide marking pen for manual macrodissection and 50% - 80% for the areas circled digitally for milling microdissection. Although a KRAS mutation was undetectable in the manually macrodissected sample, a mutation (KRAS c.35G>T, p.Gly12Val, mutant allele frequency 20%) was detected in the sample obtained by digitally guided microdissection. The top image shows the intraductal neoplasm on the H&E slide at high (20×) magnification. The bottom two images are the aligned H&E digital image and the dissection slide (stained with aniline blue) with the region for milling dissection marked digitally in green.