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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Nucl Med. 2015 Nov 5;57(2):285–290. doi: 10.2967/jnumed.115.166462

FIGURE 1.

FIGURE 1

Evaluation of binding specificity of FITC-RamAb to VEGFR-2. (A) Assessment of RamAb binding to VEGFR-2–positive HUVEC and VEGFR-2–negative A549 cells by confocal imaging. RamAb was incubated with selected cells, followed by AlexaFluor488-labeled goat antihuman IgG for confocal imaging analysis. Scale bar, 30 μm. (B) VEGFR-2 expression was further evaluated using flow cytometry, revealing large shift in fluorescence intensity for HUVEC cells as compared with A549 cells. Additionally, conjugation of RamAb to NOTA did not affect binding affinity of antibody in HUVEC cells (green). DAPI = ⋯.