Fig. 5.

Deletion of Ase1A disrupts MT network integrity in multinucleates. a MT arrays in binucleate WT (left) and Ase1A⁻ cells (right). While the two arrays are clearly distinguished in WT, centrosomes appear merged and MTs intertangled in Ase1A⁻ cells. Bar 10 μm. b Centrosomes imaged with γ-tubulin-GFP (green), and c quantification of distance between binucleate centrosome pairs, displayed as box plots. The average distance between binucleate centrosomes in WT is 6.7 ± 3.6 μm; in the knockout, average distance is 3.6 ± 1.3 μm. In Ase1A⁻ cells rescued with full-length Ase1A, the average distance is 5.4 ± 1.7 μm