Fig. 8.

GFP-Ase1A labeling during mitosis in a wild type cell. Panel shows fluorescent Ase1A live cell images on top row, and corresponding phase contrast frames on bottom row. Time 0 captures nucleolar localization prior to mitosis; 12 min panel shows redistribution of this label as the cell enters division. Note the bright label of the spindle midzone in frames 16 and 21 min. Even the spindle poles show some staining (arrowheads). As the cell begins to cleave (frame 27), most of the spindle label is concentrated in the midbody. Time in min; bar 5 μm