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. Author manuscript; available in PMC: 2017 Jan 16.
Published in final edited form as: J Mol Biol. 2015 Dec 3;428(1):26–40. doi: 10.1016/j.jmb.2015.11.025

Figure 2. PRORP2 discriminates betweeen binding, but not cleaving, substrates with varied 5′ leader and 3′ trailer lengths.

Figure 2

A. Representative plots of fluorescent polarization assays performed at 25 °C in 30 mM MOPS pH 7.8, 150 mM NaCl, 1mM DTT and 6 mM CaCl2 with varied PRORP2 (0–1000 nM) and 20 nM 5′ fluorescently labeled: (nu)pre-tRNAGly 23:10 (closed circle), (nu)pre-tRNAGly 23:5 (closed square), (nu)pre-tRNAGly 23:1 (diamond), (nu)pre-tRNAGly 13:1 (closed upside down triangle), and (nu)pre-tRNAGly 8:1 (right triangle). B. Representative single turnover assays using standard assay conditions with 5 μM PRORP2 and 50 nM nuclear fluorescently labeled (nu)pre-tRNAGly 23:10 (closed circle), (nu)pre-tRNAGly 23:5 (closed square), (nu)pre-tRNAGly 23:1 (diamond), (nu)pre-tRNAGly 13:1 (closed upside down triangle), and (nu)pre-tRNAGly 8:1 (right triangle).