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. Author manuscript; available in PMC: 2017 Jan 16.
Published in final edited form as: J Mol Biol. 2015 Dec 3;428(1):26–40. doi: 10.1016/j.jmb.2015.11.025

Figure 3. PRORP2 cleaves mitochondrial pre-nad6 t-element.

Figure 3

A. Proposed secondary structure of the (mt)pre-nad6 t-element14:6 used in our in vitro assays. B. Percentage of the (mt)pre-nad6 t-element14:6 cleaved as a function of time under single turnover conditions (50 nM 5′ fluorescein end labeled (mt)pre-nad6 t-element14:6, 5 μM PRORP2, 25°C, 30 mM MOPS pH 7.8, 150 mM NaCl, 1 mM DTT and 1 mM MgCl2).