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. Author manuscript; available in PMC: 2017 Jan 16.
Published in final edited form as: J Mol Biol. 2015 Dec 3;428(1):26–40. doi: 10.1016/j.jmb.2015.11.025

Figure 5. PRORP2 is a monomer in solution.

Figure 5

A. Residue K42 in the PPR domain and active site residues of two adjacent in crystallo PRORPs (residues D421 and D422). B. Representative plot of 5 μM K42A PRORP2 cleaving 50 nM (nu)tRNAGly8:1 in single turnover cleavage assays under standard reaction conditions. C. Analytical Ultracentrifugation experiment with 1 and 10 mg/ml PRORP2.