Table 1.
Electrophysiological characteristics in DRN-5-HT and non-5-HT neurons.
| 5-HT | Saline/saline n=7 (7 animals) |
Saline/yohimbine n=8 (6) |
Cocaine/saline n=8 (6) |
Cocaine/yohimbine n=9 (7) |
|---|---|---|---|---|
| Amplitude (pA) | ||||
| sIPSC | 14.51±1.00 | 14.16±1.17 | 16.05±2.11 | 23.43±2.12 * |
| mIPSC | 13.80±1.15 | 12.76±0.92 | 14.89±2.21 | 24.48±3.04 * |
| Frequency (Hz) | ||||
| sIPSC | 1.16±0.15 | 2.20±0.51 | 2.22±0.94 | 1.86±0.51 |
| mIPSC | 0.96±0.12 | 1.12±0.27 | 1.24±0.17 | 0.97±0.20 |
| mIPSC kinetics | ||||
| Rise time (ms) | 2.87±0.39 | 2.84±0.41 | 2.41±1.56 | 2.17±0.18 |
| Fast decay (tau) | 9.42±0.84 | 10.33±3.41 | 6.91±0.89 | 9.37±0.91 |
| Slow decay (tau) | 43.38±11.87 | 49.26±23.48 | 23.74±2.60 | 21.72±1.76 |
| Holding current (pA) | −20.86±8.63 | −23.59±8.95 | −47.56±19.24 | −36.36±10.57 |
| Non-5-HT | n=9 (7 animals) | n=6 (5) | n=8 (6) | n=6 (6) |
| Amplitude (pA) | ||||
| sIPSC | 22.02±3.04 | 31.20±6.71 | 22.27±3.96 | 23.96±2.67 |
| mIPSC | 24.56±4.30 | 25.36±5.48 | 18.04±2.21 | 23.30±2.42 |
| Frequency (Hz) | ||||
| sIPSC | 1.03±0.14 | 1.30±0.28 | 1.68±0.42 | 1.67±0.46 |
| mIPSC | 0.94±0.13 | 1.12±0.22 | 1.83±0.82 | 1.45±0.36 |
| mIPSC kinetics | ||||
| Rise time (ms) | 2.24±0.33 | 2.49±0.36 | 2.49±0.34 | 2.11±0.42 |
| Fast decay (tau) | 8.82±1.07 | 7.83±0.85 | 10.75±2.50 | 5.94±1.10 |
| Slow decay (tau) | 32.08±6.96 | 26.02±6.91 | 48.77±16.96 | 19.41±4.54 |
| Holding current (pA) | 0.21±12.20 | −18.54±13.02 | −12.38±7.94 | −9.13±17.69 |
Note: Amplitude of sIPSCs and mIPSCs was significantly elevated selectively in DRN-5-HT neurons following stress-induced reinstatement (*p<0.05, unpaired Student’s t-test). All other electrophysiological characteristics in DRN neurons were not different between treatment groups. Frequency of sIPSCs was significantly higher than that of mIPSC in DRN-5-HT neurons from saline/yohimbine group (p<0.05 paired Student’s t-test), and there was a trend of higher frequency of sIPSCs than that of mIPSC in cocaine/yohimbine group (p=0.065).