Table 8.
Development time differences in gene expression for organ pipe-reared Punta Prieta, Baja California flies comparing up/down expression differences for day 1, 2, 3, and 4 development times, all FDR P < 0.05. Comparisons indicate greater (>) and lesser (<) transcript amounts for each comparison and numbers of genes involved.
Emergence day comparison | ||||||||
---|---|---|---|---|---|---|---|---|
Day 1 vs 2 | Day 1 vs 3 | Day 1 vs 4 | ||||||
Up/down comparison+ | GOterm/ID | Enrich score | Up/down comparison+ | GOterm/ID | Enrich score | Up/down comparison+ | GOterm/ID | Enrich score |
Day 1 > 2 (1, 0) | none | Day 1 > 3 (1, 1) | Dmoj\GI19497 Gadd45; regulation of oviposition | Day 1 > 4 (4, 1) | 1. Dmoj\GI24142, endosome transport | |||
Day 1 < 2 (3, 2) | 1. Dmoj\GI21890 aconitate hydratase 2. Dmoj\GI18654 pAbp; poly(A) RNA binding |
Day 1 < 3 (5,2) | 1. CG31559; electron carrier 2. Dmoj\GI18654 pAbp; poly(A) RNA binding |
Day 1 < 4 (40, 35) | 1. Mitochondria, ATP synthesis 2. TCA cycle 3. endopeptidase activity |
4.2**** 2.7* 0.2 |
||
Day 2 vs 3 | Day 2 vs 4 | Day 3 vs 4 | ||||||
Up/down comparison+ | GOterm/ID | Enrich score | Up/down comparison+ | GOterm/ID | Enrich score | Up/down comparison+ | GOterm/ID | Enrich score |
Day 2 > 3 (6, 4) | 1. Dmoj\GI19844; exu, bicoid mRNA localization 2. desat2 3. Dmoj\GI23403; triglyceride lipase 4. Dmoj\GI18075; ATP synthesis |
Day 2 > 4 (1, 0) | FBtr0162777 (not annotated) | Day 3 > 4 (31, 14) | 1. nucleotide binding | 1.5 | ||
Day 3 > 2 (2, 2) | unknown | Day 4 > 2 (65,49) | 1. mitochondria 2. mitochondria, phosphorylation 2. metal ion binding |
1.4 1.0 0.2 |
Day 4 > 3 (95, 59) | 1. ATP synthesis 2. signal peptide |
1.3 0.5 |
numbers in parentheses denote (number of genes, number of genes with D. melanogaster orthologs)