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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Cancer Res. 2015 Nov 25;76(3):582–593. doi: 10.1158/0008-5472.CAN-15-1199

Figure 4. Profound impact of PROX1 activity to the transcriptional profiles of thyroid cancer cells.

Figure 4

(A) Venn diagram showing that PROX1 regulates approximately 10% of differentially regulated genes in the normal tissues vs. matched PTC. Genes with > 2-fold changes were selected in each data base. (B,C) IPA-based analyses identified Wnt/β-catenin signaling as a key pathway that was suppressed in PTC tissues from two independent PTC expression studies (GSE3467, GSE3678). Genes that were previously associated with Wnt/β-catenin signaling are listed with their expression changes. (D) PROX1 re-expression enhances the activity of Wnt/β-catenin signaling in BCPAP cells. The β-catenin activity reporter vector (TOP-Flash) or its mutant control vector (FOP-Flash) was transfected for 12 hours and the cells were treated with or without Dox for 36 hours. (E,F) Examples of genes are listed whose expression was consistently changed in PTC tissues (E) Expression of these genes was correspondingly regulated by PROX1 re-expression in BCPAP cells (F). Error bars present standard deviations. *, p < 0.05; **, p < 0.01; ***, p < 0.001.