Figure 7.

Quinine does not reveal the functional contribution of TWIK-1 and TREK-1 in single or double gene knockout mice. (A) Representative astrocyte V_M recordings first in 100 μM BaCl₂ bath application for 5 min, followed by addition of 400 μM quinine for 20 min, from a WT, TREK-1^−/−, TWIK-1^−/− and TWIK-1^−/−/TREK-1^−/− astrocyte as indicated in situ. (B) Summary of 400 μM quinine-induced V_M depolarization (ΔV_M 1) and the total V_M depolarization induced by BaCl₂ plus quinine from all four genotypes. (C) Representative whole-cell current recordings first in aCSF as control, then in 100 μM BaCl₂ plus 400 μM quinine, and washout. Representative I–V relationships were shown in the right panel. (D) Summary of RI values from four genotypes obtained from astrocyte recordings in the presence of 400 μM quinine and 100 μM BaCl₂ together in bath. The RI values were comparable among the four genotypes.