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. 2016 Feb 3;6:20352. doi: 10.1038/srep20352

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(A) Immunoblot analysis of ERα (HA-tagged) and tau in M17 cells transiently transfected with indicated vectors. (B) Tau overexpression reduces E2-stimulated transcriptional activity of ERα by 30%. The ERE reporter luciferase activity was normalized against the control renilla luciferase activity to correct for transfection efficiency. All values were presented as the fold induction over the control luciferase activity in the untreated vector cells, which was taken as 1. Data are means ± s.e.m of three independent experiments. **p < 0.01, ***p < 0.001. One way ANOVA with Newman-Keuls test.