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. 2016 Feb 3;6:20033. doi: 10.1038/srep20033

Figure 1. Acquisition and identification of forebrain-like neurons from iPS.

Figure 1

(a), Immunostaining showed iPS-derived NPCs expressing Pax6, Sox1 and Nestin of high percentages while no expression of pluripotent marker Oct4 (b), which was further confirmed by Q-PCR, (c), however, the mesoderm marker (T) and endoderm marker (Sox17) were undetectable in these NPCs. (d), Q-PCR also confirmed the dorsal forebrain identity of the NPCs, which remained the same characteristics even after long-term passages. Pax6 and Emx2 (not done in H1 NPC): markers for dorsal CNS (central nervous system), Otx2 (not done in UC5 NPC P9) and Foxg1: forebrain markers, En1 and Lmx1b: midbrain markers, Gbx2 and Hoxb2: hindbrain markers. P2, Passage 2; P9, Passage 9. (e), Immunoflurescent photographs showed the NPCs were highly neurogenic with high expression of typical neuronal markers (early Tuj1 and mature Map2). D1: 1 day post-differentiation; D24: 24 day post-differentiation.