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. 2016 Feb 3;6:20301. doi: 10.1038/srep20301

Figure 4. Hsp70 inhibition changes cell cycle distribution of HeLa cells.

Figure 4

The application of 1 induces G1 arrest in HeLa cells. HeLa cells were preincubated for 1 h at 37 °C in presence of 0.4% DMSO or 25 μM 1. Following this, cells were treated with thymidine, nocodazole or diluent for 20 h before the harvest. The HeLa cells were analyzed for the DNA content by flow cytometry. The positions of the G1 (1) and G2/M (2) peaks are marked. The experiment was performed three times with the same results.