Table 1. Inhibition of Hsp70 assisted refolding of firefly luciferase by 9-aminoacridizinium derivatives.

^aCharacterization of the compounds including NMR data can be found in the literature⁴⁴,⁴⁶,⁶⁵.

^bIC₅₀ values were determined as described in Methods. Briefly, reactivation of GdmCl denatured luciferase (2 μM) was induced by dilution 1:200 in 800 nM Hsc70 and 200 nM DnaJ or 1500 nM Hsp70 und 1000 nM DnaJ in renaturation buffer (10 mM MOPS, pH 7.8, 50 mM KCl, 5 mM ATP, 5 mM MgCl₂, 5 μM BSA) in the presence of increasing concentrations of 9-aminoacridizinium bromide derivatives at 25 °C. After 2 h of renaturation the activity of luciferase was determined. IC₅₀ value determinations were based on duplicate eight-point dose response curves. The mean IC₅₀ and standard error of the measurements were calculated via nonlinear regression.