Table 2. Percent DNs expressing each neurotransmitter.

Neurotransmitter	Labeled using	Percent
Acetylcholine	Cha-Gal4 > GFP (neurons expressing choline acetyltransferase)	38
GABA	GABA antibody	37
Glutamate	VGlut-Gal4 > UAS-mCD8-GFP (neurons expressing vesicular glutamate transporter)	6*
Serotonin	Ddc-Gal4 > UAS-mCD8-GFP (neurons expressing dopamine decarboxylase)	3*
Octopamine	Tdc2-Gal4 > UAS-mCD8-GFP (neurons expressing tyramine decarboxylase)	1*
Dopamine	TH antibody	0.2*
Total		85

For acetylcholine and GABA, the percentage was computed by summing the largest number of colabeled neurons in each cluster, then dividing by the total number of bulk labeled neurons found in those clusters (see Supplementary Data for details).

^*Percent for neuromodulators reported as the result of dividing the sum of the maximum number of colabeled neurons per cluster by 900, the average number of neurons labeled by our bulk labeling technique.