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. 2016 Feb 3;6:20237. doi: 10.1038/srep20237

Table 1. Incubation conditions with various staining agents that have been used to identify the plasma membrane, endosomes, mitochondria, lipid droplets and lysosomes.

Cell line Staining agent Incubation Excitation wavelengths (λ, nm) Emission Wavelengths (λ, nm)
MA-104 Fluorescein-Lectin WGA 0.02 mg.mL−1, 10 min 488 499–535
Rhodamine-Lectin WGA 0.02 mg.mL−1, 10 min 543 557–627
Nile-Red 600 nM, 15 min 543 590–697
Mitotracker deep red 200 nM, 25 min 633 683–817
LysoTracker deep red 100 nM, 30 min 633 675–817
Caco-2 Fluorescein-Lectin WGA 0.02 mg.mL−1, 30 min 488 499–535
Rhodamine-Lectin WGA 0.02 mg.mL−1, 30 min 543 557–627
LysoTracker deep red 100 nM, 30 min 633 675–817