Figure 2. Fluorescence spectra of mutant thaumatin proteins.
Plant thaumatin (black circle), D21N (red circle), E42Q (purple dot), D55N (blue triangle), D59A (green diamond), D60A (cyan diamond), E89Q (yellow square). Plant thaumatin denatured by 6 M urea (black dot) were excited at 280 nm and emission spectra were recorded at 25 °C.