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. 2016 Feb 3;6:20282. doi: 10.1038/srep20282

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(a) Expression of hTPC2 in DT40TKO-EGFP (lane 1) and DT40TKO-hTPC2 (lane 2) cells. Twenty-five μg of cell lysate were loaded in each lane; arrowhead indicates the immunoreactive band of TPC2 protein. (b) Fluorescent microscopy of GFP-tagged hTPC2 protein in intact and ruptured DT40TKO-hTPC2-GFP cells counterstained with ER tracker. Arrow indicates the expression of GFP-tagged hTPC2 protein on the nuclear envelope of a ruptured DT40TKO cell.