Skip to main content
. 2016 Feb 3;6:20282. doi: 10.1038/srep20282

Figure 5. NAADP elicited biphasic regulation of the hTPC2 channel expressed on DT40TKO-hTPC2 nuclei.

Figure 5

(a) Representative hTPC2 single-channel current traces activated by various NAADP concentrations, as tabulated, at +60 mV. No current was detected when NAADP was excluded from the pipette solution (n = 20 patches). The NAADP-activated single-channel current was inhibited when 1 μM Ned-19 was included in the pipette solution (n = 20 patches). Arrows indicate zero current level. (b) Relationship between hTPC2 Po and [NAADP]. All data were recorded at +60 mV and data were summarized as mean ± SEM from 3 individual recordings at each [NAADP]. (c) Summary of effects of [NAADP] on hTPC2 channel mean open and mean closed times. Data were compared to 1 nM NAADP by one-way ANOVA with Dunnett’s post hoc test. Individual dwell time analysis at different [NAADP] was presented in Supplementary Fig. 3.