Figure 2. RRM proteins exhibit distinct binding abilities of ssDNA in a sequence-specific manner.

(A) A schematic representation of a pull-down assay using His-tagged RRM proteins and Ni²⁺-affinity resins. (B–D) The molar ratio between ssDNA and RRM proteins (RRM1, RRM2 and RRM1–2 in (B–D), respectively) in a pull-down assay was plotted against NN′, which describes a repeating part in an ssDNA sequence, 5′-A₁₀(NN′)₅A₁₀-3′. An exception is TT, which is an ssDNA with the sequence, 5′-C₁₀(TT)₅C₁₀-3′. More than three independent experiments were performed to estimate error bars (standard deviations).