Figure 1. ATP hydrolysis-impaired MukB^EQEF complexes associate with ter.

Localization of ori1, ter3, and MukB-mYPet (SN182) (a) or MukB^EQ-mYPet (SN311) (b). The ori1 locus is a lacO array bound by LacI-mCherry, and the ter3 locus is a tetO array bound by TetR–CFP. The normalized fluorescence intensity along the cell length is shown below each image. (c) Left panel: % colocalization at 2 pixels between MukB, MukB^EQ, ori1 and ter3 focus centroids in the combinations shown. Dashed boxes represent the distances measured to a random pixel position in the cells analysed. The histograms show the mean (±s.d.) of three independent experiments. Middle and right panels show cumulative distributions of the distances between MukB, or MukB^EQ, and ori1 or ter3 foci, respectively, obtained in one experiment. Dashed lines represent distances measured to a random pixel. The grey rectangle indicates the distances within 2 pixels (258 nm). See also Supplementary Figs 1 and 2. CFP, cyan fluorescent protein).