Table 1. Primary antibodies.
| Antigen | Primary antibody | Species raised in | Dilution | Fixation | Company |
|---|---|---|---|---|---|
| Aβ | Human Aβ (715800) | Rabbit | 1:100 | Acetone | Invitrogen, Camarillo, CA, USA |
| tTG | Guinea pig tTG (06471) | Goat | 1:4000 | Acetone | Millipore, Temecula, CA, USA |
| Astrocytes | Bovine Glial Fibrillary Acidic Protein | Rabbit | 1:4000 | Acetone | DAKO, Glostrup, Denmark |
| Microglia | Iba-1 (019–19741) | Rabbit | 1:500 | PFA | WAKO Chemicals, Richmond, VA, USA |
Abbreviations: Aβ = amyloid beta, Iba-1 = Ionized calcium binding adaptor molecule 1, PFA = paraformaldehyde, tTG = tissue transglutaminase.