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. 2016 Feb 3;6:20564. doi: 10.1038/srep20564

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Recognition and counting of cancer cells with DAPI staining in CK stained area, as green pseudocolor (A1,A2) and cancer cells with Ki67 staining in CK stained area as cyan pseudocolor (B1,B2) both by the developed computerized method; the original image of CK, Ki67 and DAPI imaging for BC (C1) and merged image of recognition and counting of cancer cells with DAPI staining and Ki67 staining (C2). Stromal cells marked with green arrows (A1,A2, C1 and C2) and stromal Ki67-positive cells marked with cyan arrows (B1, B2, C1 and C2) were not recognized by the computerized method. (Magnifications: 400×).