Table 1. Summary of knock-in animal production.

Nuclease	RS-1	Target	No. of embryos transferred	No. of kits	Kits with indels but no KI	Kits with KI	KI per embryo (%)	KI per kit (%)
TALEN	−	rbApoAI	227	16	11	1	0.4	6.3
TALEN	+	rbApoAI	145	17	7	3	2.1*	17.6
Cas9	−	RLL	373	43	29	3	0.8	7.0
Cas9	+	RLL	146	38	18	10	6.8**	26.3**

Cas9, CRISPR-associated protein 9; indels, insertion and deletions; KI, knock-in; TALEN, transcription activator-like effector nuclease.

Comparisons were made between RS-1 supplemented group (+) and non-treatment group (−) of the same locus. *P<0.05, **P<0.01.