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. 2016 Feb 3;6:20566. doi: 10.1038/srep20566

Figure 3. Generation and identification of B. bassiana vlp mutants.

Figure 3

(A–C) Diagrams for the deletion of vlp1, vlp2 and vlp3 from WT, respectively, and their mutants identified by PCR (lanes 1–3) and Southern blotting (lanes 4–6) analyses with paired primers and amplified probes (Table S1). Lanes 1 and 4: WT. Lanes 2 and 5: Δvlp mutant. Lanes 3 and 6: Δvlp::vlp mutant. The genomic DNAs of the vlp1, vlp2 and vlp3 mutants used in Southern blotting hybridization were digested with ClaI/EcoRI, EcoRI/EcoRI and ClaI/ClaI, respectively.