Figure 6. Maintained insulin sensitivity and circulating lipid and hormone profile in MC3R^hDM/hDM mice.

Serum samples were used to measure (a) triglycerides, (b) total cholesterol, (c) glucose, (d) insulin, (e) free fatty acids and (f) corticosterone in 3-month-old female MC3R^hWT/hWT (open symbols) and MC3R^hDM/hDM (closed symbols) mice. For MC3R^hWT/hWT n=8 except n=6 for triglycerides and corticosterone; for MC3R^hDM/hDM n=9. For glucose (g) and insulin (h) challenge tests (n=7 per group), glucose and insulin were injected at time 0. Fat mass (i) and fat-free mass (j) were determined on the day of the glucose tolerance test. Data are represented as mean±s.e.m. A different letter represents significant differences at P<0.05 compared to the other groups. Groups were compared with one-way (a–f) and two-way (g–h) analysis of variance followed by Bonferroni post-tests and Student's t-tests (two-tailed) (i,j).