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. 2015 Oct 30;46(1):167–177. doi: 10.1002/eji.201444970

Figure 1.

Figure 1

PRR signaling in human MDDCs reduces miR‐650 expression. Human MDDCs were treated with the indicated (A) TLR, (B) NOD‐like receptor or (C) RIG‐like helicase ligands for 24 h. miR‐650 expression was measured by qRT‐PCR. (D) Stimulation of MDDCs with 0.1, 1, or 10 μg/mL ssRNA40 for 24 h. miR‐650 expression was measured by qRT‐PCR. (E) Stimulation of MDDCs with ssRNA41 (10 μg) or LyoVec for 24 h. miR‐650 expression was measured by qRT‐PCR. (F and G) MDDCs were pretreated with 50 μM chloroquine or 30 μg/mL mianserin hydrochloride for 30 min prior to addition of (F) 10 μg/mL ssRNA40 or (G) 1 μg/mL LPS for 24 h. miR‐650 expression was measured by qRT‐PCR. For all figures, miR‐650 expression was normalized against RNU48 and is shown relative to (A–E and G) unstimulated MDDCs or (F) relative to cells incubated with LyoVec. All measurements were performed in triplicate and data are shown as mean ± SEM and are pooled from (A, B, D, and E) four, (F and G) three, or (C) two individual donors. Statistical analysis was performed using a paired Student's t‐test (*p < 0.05).