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. 2016 Jan 18;212(2):181–198. doi: 10.1083/jcb.201506081

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© 2016 Liu et al.

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Figure 3. — Loss of sorf-1 and sorf-2 delays early-to-late endosome conversion. (A–D) Time course of dynamic changes of PtdIns3P (2xFYVE::GFP) and RAB-7 (mCherry::RAB-7) on endosomes in N2 (A), sorf-1(tm3855) (B), sorf-2(tm5210) (C), and sorf-2(tm5210);sorf-1(tm3855) (D) coelomocytes. *, Time points at which endosomes reach peak sizes. White and yellow arrows indicate the chased endosomes before and after enrichment of RAB-7, respectively. Bars, 5 µm. (E) Duration of 2xFYVE::GFP on endosomes. ≥18 endosomes were examined for each genotype. (F) Rate of disappearance of 2xFYVE::GFP-positive endosomes. ≥12 coelomocytes were examined. (G) Peak sizes of endosomes (n > 100) in A–D. Each symbol represents an endosome that was followed. ***, P < 0.001; NS, no statistical significance.