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. 2016 Jan 18;212(2):181–198. doi: 10.1083/jcb.201506081

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© 2016 Liu et al.

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Figure 6. — PI3K-dependent persistence of PtdIns3P leads to continuous fusion of early endosomes. (A–C) Time-lapse analysis of RAB-7(T23N)-induced fusion of 2xFYVE::GFP-labeled early endosomes in N2 (A), sorf-1(tm3855) (B), and sorf-2(tm5210) (C) coelomocytes. Colored arrows indicate different endosomes that were followed. (D) Time-lapse images of RAB-7(T23N)-induced fusion of early endosomes labeled with GFP::RAB-5. (E) Time-lapse images of RAB-7(T23N)-induced fusion of early endosomes labeled with GFP::RAB-5 in vps-34 RNAi–treated sorf-1 and sorf-2 mutants. Bars, 5 µm.