Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 18;212(2):181–198. doi: 10.1083/jcb.201506081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Liu et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 9. — WDR91 and WDR81 act in complex with Beclin1 to affect endosomal PtdIns3P levels. (A) Immunostaining of endogenous EEA1 in control (Ctrl), KO-81, and KO-91 HeLa cells treated with control siRNA (siCtrl) and Beclin1 siRNA (siBeclin1; left). Boxed regions are magnified (2×) in insets. Bars, 15 µm. Bottom left: EEA1 intensity on endosomes (arbitrary units [AU]) in ≥100 cells. ***, P < 0.001. (bottom right) Western blot of Beclin1 and EEA1 in cells treated with siBeclin1 or siCtrl. (B) Knockout of WDR91 (KO-91) or WDR81 (KO-81) increases the sizes of early endosomes in HeLa cells in a Beclin1-dependent manner. Cells were imaged 24 h after transfection with 2xFYVE-mCherry expression vector ± siBeclin1. Bars, 5 µm. (C) Colocalization (arrows) of WDR81 or WDR91 with Beclin1 in immunostained cells. Insets show magnified (1.8×) views of boxed areas. Bars, 5 µm. (D–G) Co-IP of HA-Beclin1 with Flag-WDR81 (D), HA-Beclin1 with Myc-WDR91 (E), Flag-Vps34 with Myc-WDR81 (F), and Flag-Vps34 with Myc-WDR91 (G). Flag or Myc antibodies were used for IPs. Precipitated proteins were detected with HA, Myc, or Flag antibodies.