Figure 8.

Model for a role of Nup60 ubiquitylation in the plasticity of the nuclear basket and its functional consequences. Nup60 is anchored to the nuclear membrane via its N-terminal amphipathic helix (Mészáros et al., 2015) and to the core NPC by the interaction of ubiquitylated Nup60 to Nup84. The combination of both anchoring pathways promotes stable association of Nup60 and Nup2 at the NPC required for an efficient DDR. In contrast, preventing ubiquitylation of Nup60 induces a more dynamic association and dissociation of both Nup60 and Nup2 with the NPC, likely resulting in a partially unstable nuclear basket (dashed fibrils) and altered DDR. The scaffold of this scheme has been adapted from Mészáros et al. (2015).