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. 2016 Jan 18;212(2):219–229. doi: 10.1083/jcb.201508056

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© 2016 Wieschaus and Weng

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Figure 5. — Myosin is required for the junctional remodeling and ectopic activation of myosin remodels junctions in a manner recapitulating that of mesodermal cells. (See also Fig. S4.) (A and B) Strong myosin RNAi (sqh RNAi) leads to loss of adherens junctions. Bar, 20 µm. (A′ and B′) Zoom-in images of the ventral cells. Bar, 20 µm. (C) Comparison between wild-type and moderate myosin RNAi (RNAi against zip) embryos on the time required for the junction shift. n = 5 for wild-type and n = 4 for zip RNAi. (D) Kymographs of E-Cad::GFP in ventral cells of wild-type and zip RNAi embryos. (E) Kymographs of E-Cad::GFP and Sqh::mCherry in dorsal cells with ectopic myosin contraction. (F) Mean correlation coefficients between myosin activity and junctional features in dorsal cells expressing Fog. The intensity of myosin including junctional and nonjunctional myosin is used here for correlation analysis. n = 26. (G and H) Arm staining in a wild-type (G) and a kr>fog (H) embryo. G and H are midsagittal sections of dorsal cells. G′ and H′ are max projections of the lateral surface of the embryos. Bars, 20 µm. β-Cat, β-catenin; AJ, adherens junctions; Myo, myosin.