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. 2015 Dec 12;38(1):27–40. doi: 10.1002/bies.201500104

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Published 2015. This article is a U.S. Government work and is in the public domain in the USA. Bioessays published by WILEY Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Approximate maximum‐likelihood phylogenetic tree of the Ime‐4/MT‐A70 methylase clade generated using the FastTree and MEGA5 programs. Proteins are labeled using species abbreviations and gi, and colored based on their phylogenetic position in the eukaryotic tree (shown on left). Bootstrap values for major branches of the tree are shown. Related bacterial subclades from which the Ime‐4/MT‐A70 MTases were derived form successive outgroups to the eukaryotic subclades. Species abbreviations for all figures: Aano, Aureococcus anophagefferens; Acas, Acanthamoeba castellanii; Aque, Amphimedon queenslandica; Atha, Arabidopsis thaliana; Bden, Batrachochytrium dendrobatidis; Bnat, Bigellowiella natans; Bmal, Brugia malayi; Ccin, Coprinopsis cinerea; Ccor, Conidiobolus coronatus; Cele, Caenorhabditis elegans; Cflo, Camponotus floridanus; Cmer, Cyanidioschyzon merolae; Cowc, Capsaspora owczarzaki; Crei, Chlamydomonas reinhardtii; Csub, Coccomyxa subellipsoidea; Ddis, Dictyostelium discoideum; Dmel, Drosophila melanogaster; Drer, Danio rerio; Ehux, Emiliania huxleyi; Esil, Ectocarpus siliculosus; Glam, Giardia lamblia; Hsap, Homo sapiens; Lmaj, Leishmania major; Mbre, Monosiga brevicollis; Mver, Mortierella verticillata; Ncra, Neurospora crassa; Ngru, Naegleria gruberi; Nvec, Nematostella vectensis; Otau, Ostreococcus tauri; Otri, Oxytricha trifallax; Pfal, Plasmodium falciparum; Pmar, Perkinsus marinus; Ppal, Polysphondylium pallidum; Ppar, Phytophthora parasitica; Ppat, Physcomitrella patens; Ptet, Paramecium tetraurelia; Rfil, Reticulomyxa filose; Rirr, Rhizophagus irregularis; Rmic, Rhizopus microspores; Scer, Saccharomyces cerevisiae; Sinv, Solenopsis invicta; Spar, Saprolegnia parasitica; Spom, Schizosaccharomyces pombe; Slem, Stylonychia lemnae; Spun, Spizellomyces punctatus; Sros, Salpingoeca rosetta; Tgon, Toxoplasma gondii; Tpse, Thalassiosira pseudonana; Tthe, Tetrahymena thermophila; Tvag, Trichomonas vaginalis; Vcar, Volvox carteri.