Table 1. Changes in the metabolite concentrations following mock inoculation and PVY inoculations.

Metabolic pathway	Metabolite	Time post infection (dpi)
		1		3		6
		N:mock	NTN:mock	N:mock	NTN:mock	N:mock	NTN:mock
Gaba shunt	GABA	-0.51	-0.57	-0.11	0.09	1.11*	1.47*
	Glycerate	-0.99	-1.13*	0.31	0.02	0.87*	1.22**
Amino-acid synthesis	Citramalate	-0.20	-0.70	0.25	0.52	0.29	0.66*
	Glutamate	-0.20	-0.13	0.16	-0.22	0.64	1.16*
	Phenylalanine	-0.17	-0.33	0.19	0.02	1.28**	2.11***
	Shikimate	-0.50	-0.33	0.61	0.47	0.42	0.55*
	Tryptophan	-0.02	-1.67	-0.25	-0.66	1.64	2.23*
	Valine	-0.05	-0.46	-0.08	-0.99	2.26**	4.42*
	Threonine	0.33	-0.10	-0.02	-0.42	0.31	0.85*
Major carbohydrate	Fructose-6-P	-1.60	-1.78	-0.03	-0.69	1.83	1.68*
	Glucose-6-P	-1.86	-1.97	-0.09	-0.81	1.81	1.66*
	Maltose	-1.13	-0.83	0.33	-0.88	2.89*	3.18*
	Sucrose	-0.43*	-0.45*	0.31	0.00	0.82*	0.83*
TCA cycle	2-Oxoglutarate	-1.09	-1.66	1.18	0.05	3.44*	3.70**
	Citrate	-0.48	-1.15	0.68*	1.23	0.18	1.34
	Succinate	-0.52	-0.52*	0.20	0.31*	0.88*	1.04***
	Pyruvate	-0.01	-0.53	0.54	0.54	1.27	1.76**
ROS scavengers	Dehydroascorbate	-0.36	-0.27	0.67	-0.38	0.94	0.98*
	Lyxonate	-0.82	-0.99	0.18	-0.32	1.59	1.78**
	Mannose-6-P	-1.86	-1.90	-0.06	-0.81	1.78	1.55*
	Pyroglutamate	-0.42	-0.57	0.02	-0.07	0.24	0.41*
	Threonate	-0.96	-1.22*	0.31	0.00	1.38	1.86*
Phenylpropanoid	4-Caffeoylquinate-trans	-1.98	-4.44	0.15	-0.67	2.39	2.83**
	Chlorogenate-trans	-1.56	-3.00	0.37	-0.53	2.58	2.91**

The responses in the leaves to inoculation with PVY^N and PVY^NTN are compared to the mock inoculation (N:mock, NTN:mock, respectively). The log2 ratios of the metabolite concentrations at the different times (i.e., 1, 3, 6 dpi) are shown. The corresponding metabolic pathways were assigned. The statistical significances of the changes in metabolite levels were evaluated (t-tests).

*, p <0.05;

**, p <0.01;

***, p <0.001.