Table 3. Target sequences, forward (FP) and reverse (RP) primers, and probe sequences (Probe) of amplicons for the genes investigated.
| Gene | Target sequence ID | Primer sequence (5’– 3’) | Amplicon length(nt) | Amplification efficiency(%) | Source |
|---|---|---|---|---|---|
| BAM | AF393847a; Sotub08g023010 b | FP: TGTAAAGATGTGTCAAGAACATGGATT; RP: AATCTCCAACATTTCCTCCACACT; Probe: AGCTTCAAGTTGTCATGTCTTTTCA | 80 | 83 | This study |
| FRK | BQ510934 a; Sotub03g007180 b | FP: TGCCCTGACAGTGACAGGAA; RP: GCAGTGACCTCGGCAAGAGT; Probe: TCCCATCCCTTCCTACACAAGATGCA | 84 | 112 | This study |
| HKS | AF106068 a; Sotub04g034770 b; Sotub11g020550 b | FP: GTCTTTTATGCATTGGATCTTGGT; RP: ACCATCTTTTCCMCCCAATT | 72 | 89 | This study |
| GAD | BQ514644 a; Sotub11g007320 b | FP: TGGAGTTGGAACAGTTGGATCTT; RP: GCTTTCATTTTGTTTTGCCATTT; Probe: AGCCATTATGCTTGCTGGATTGGCCTTT | 83 | 92 | This study |
| SSADH | TA26508_4113 d;Sotub09g025220 b | FP: GCAGATCTTGAAGTAGCTTTAAAAGGA; RP: TCGCGCATACACATGTTTGTC; Probe: CTCTGGCAACAAAGTTCCGTAACACC | 76 | 94 | This study |
| PAL | MICRO.2219.C3 c; Sotub09g007470 b | FP: CCTCCGTGGMACGVTCAC; RP: TGAGCAAACCAGCAATGTAGGA; Probe: CCTCRGGTGATCTTGTCCCTC | 65 | 89 | This study |
| C4H | TA25065_4113 d; Sotub06g033350 b | FP: TTGCAAATACCAAGAGCATGGA; RP: TCCCTTCTGTTG AGCATCAAGA | 77 | 99 | This study |
| CWINV | STMIM75TV c; Sotub10g025820.1.1 b | FP: CGCGGAGAGAATCACAATTGA; RP: TCTCCCAATGTTCTAGTGCAACTTT; Probe: CTAAATGCTTGGAGCATGGCTAATG | 75 | 102 | [52] |
| EF-1 | MICRO.37.C4 c; MICRO.37.C14 c; Sotub06g010680.1.1 b | FP: GGAAGCTGCTGAGATGAACAAGA; RP: CTCACGTTCAGCCTTAAGTTTGTC; Probe: TCATTCAAGTATGCCTGGGTGCT | 72 | 91 | [8] |
| COX | X83206 a; Sotub04g015050 b | FP: CGT CGC ATT CCA GAT TAT CCA; RP: CAA CTA CGG ATA TAT AAG AGC CAA AAC TG; Probe: TGC TTA CGC TGG ATG GAA TGC CCT | 74 | 99 | [53] |
| PVYuni | AJ390300 a | FP: CATAGGAGAAACTGAGATGCCAACT; RP: TGGCGAGGTTCCATTTTCA; Probe: TGATGAATGGGCTTATGGTTTGGTGCA | 73 | 100 | [24] |
BAM, β-amylase; FRK, fructokinase; HKS, hexokinase; GAD, glutamate decarboxylase; SSADH, succinic semialdehyde dehydrogenase; PAL, phenylalanine-ammonia lyase; C4H, cinnamate 4-hydroxylase; CWINV, cell-wall invertase; EF-1, elongation factor 1; COX, cytochrome oxidase; PVYuni, Potato virus Y universal amplicon. Sequences are available on
a NCBI,
c POCI database;