Skip to main content
. 2015 Dec 30;10(12):e0145654. doi: 10.1371/journal.pone.0145654

Fig 8. D4.7R forms a complex with Cullin3.

Fig 8

HEK293T cells were transiently transfected as indicated. 48 h post-transfection, cells were harvested and lysed. 5% of the lysate was used for IB to verify expression of HA-tagged receptors, Cmyc Cullin3 and Etag KLHL12 (left panels). The rest of the lysate was subjected to IP with anti-HA (16B12). Specific purification of the receptor after IP was confirmed upon IB with rat anti-HA (1:2000). Interaction of Etag KLHL12 with D4R was verified by IB with anti-Etag (1:2000) and the presence of Cmyc Cullin3 was visualized with anti-Cmyc (right panels, 1:1000).