Figure 3.

Evaluation of untreated (control) and treated with Baicalein (500 μg ml⁻¹) or Monolaurin (500 μg ml⁻¹) biofilm of Borrelia burgdorferi B31 strain (a) and Borrelia garinii CIP103362 strain (b) after 72 h as a qualitative measurement using LIVE/DEAD BacLight staining; green – live biofilm, red – dead biofilm, images taken at 4 × magnification. Immunofluorescence staining with primary antibodies against untreated B. burgdorferi B31 strain (c) and B. garinii CIP103362 strain (d) after 72 h using AlexaFluor‐488 as a secondary antibody respectively; images taken at 10× (right) and 63× (left) magnification.