Table 1.
Antibodies/technique used | Supplier | Reference number |
---|---|---|
Primary | ||
FACS | ||
CD31 | Beckman coulter | IM2409 |
CD34 | Beckman coulter | A07776 |
CD45 | Beckman coulter | A07783 |
CD73 | BD pharmingen | 550257 |
CD146 | Biocytex | 5050-F100T |
Immunofluorescence | ||
Vimentin | Sigma | V5255 |
Calponin | Sigma | C2687 |
α-SMA | Sigma | C6198 |
Nestin | Abcys | VMA5326 |
Desmin | Sigma | D1033 |
HNF6 | Santa Cruz | sc-13050 |
HNF4α | Santa Cruz | sc-8987 |
HNF3β | Santa Cruz | sc-6554 |
HNF1α | Santa Cruz | sc-135939 |
α-foetoprotein | Santa Cruz | sc-8399 |
Albumin | Cedarlane | CL2513A |
Immunohistochemistry | ||
Albumin | Calbiochem | 126584 |
OTC | Sigma | HPA000243 |
Secondary | ||
Donkey anti-rabbit Alexa Fluor 488 | Invitrogen | A21206 |
Donkey anti-rabbit Alexa Fluor 568 | Invitrogen | A10042 |
Donkey anti-goat Alexa Fluor 488 | Invitrogen | A11055 |
Donkey anti-mouse Alexa Fluor 488 | Invitrogen | A21202 |
Donkey anti-mouse Alexa Fluor 568 | Invitrogen | A10037 |
Anti-rabbit (HRP) | Dako | K4003 |