Fig. 3.
EPR spectra of wild-type hAOX1. Experimental continuous-wave EPR spectra of dithionite-reduced wild-type hAOX1 samples at pH 7.0 (trace a) together with the corresponding simulation (trace b). For simulation parameters, see Table 2. The flavin semiquinone and MoV were not detected under these experimental conditions and therefore neglected in all of the simulations: a, wild-type hAOX1; b, simulation of complete spectrum; c, simulation of FeSI; d, simulation of FeSII. Experimental conditions: T = 20 K; microwave power, 1 mW; microwave frequency, 9.385 GHz; modulation amplitude, 0.5 mT; modulation frequency, 100 kHz.