Fig. 3.
Active transcription persists 5 h after TF-induced nuclear entry, in both stably- and transiently-bound bZIP1 targets. A comparison of the overlap between actively transcribed bZIP1 targets captured from 4tU-labeled fractions (this study), and the previously reported classes of bZIP1 targets identified from total RNA [6]. Significant overlaps of induced genes (Class IIA & IIIA) and bZIP1-repressed genes (Class IIB & IIIB) are highlighted in yellow and blue, respectively. Intersects were performed using the “Genesect” function in VirtualPlant [30]. The significance of the overlaps from bZIP1 targets captured from 4tU-labeled fractions were verified using a hypergeometric test with Class IIA (stably-bound bZIP1 induced, p = 2.2e−22), Class IIIA (transiently-bound bZIP1 induced, p = 6.8e−50) and Class IIIB (transiently-bound bZIP1 repressed, p = 2.2e−20) targets