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. 2016 Feb 3;35:25. doi: 10.1186/s13046-016-0300-8

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© Zhao et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The relative expressions of MEKK1mRNA and protein in MCF-7 and MCF-7/ADR cells after miRNA transfection. a qRT-PCR showing the mRNA expression of MEKK1 in MCF-7and MCF-7/ADR cells. b Western blot and densitometric analysis showing the protein expression of MEKK1 in MCF-7 cell and MCF-7/ADR cell. c The stability assay of MEKK1 mRNA. MCF-7/ADR cells were harvested at 0, 2, 4, 6 and 8 h after treatment with 5 mg/mL actinomycin D. Residual mRNAs was detected by qRT-PCR. All graphs show means ± S.D. of three independent experiments. (* P < 0.05 vs. NC;^# P < 0.05 vs. Single)