Fig. 8.

Overexpression of MEKK1 restored the inhibition of P-gP induced by miR-302. a Cells were co-transfected with miRNA mimics indicated in the figure and MEKK1- cDNA(lacking the 3′UTR sequence ) for 48 h, then lysates were harvested and subjected to immunoblotting with anti-MEKK1, anti-ERK, anti-p-ERK, and anti-P-gP antibodies. GAPDH was applied as control for equal loading. All experiments were carried three times independently. b Densitometric analysis for the detected protein expression. (* P < 0.05 vs. NC)