Table 1.
Gene | Primer sequence (forward/reverse) | T (annealing) | Cycles |
---|---|---|---|
OPG | 5′-TCCTGGCACCTACCTAAAACAGCA-3′ 5′-CTACACTCTCGGCATTCACTTTGG-3′ |
57 | 35 |
| |||
RANKL | 5′-ATGATGGAAGGCTCATGGTTG-3′ 5′-TGTTGGCGTACAGGTAATAGAA-3′ |
60 | 35 |
| |||
M-CSF | 5′-AAGCAGTAACTGAGCAACGGG-3′ 5′-CCCATATTGCGACACCGAA-3′ |
59 | 30 |
| |||
IL-6 | 5′-GAAATGAGAAAAGAGTTGTGC-3′ 5′-ATTGGAAATTGGGGTAGGAAG-3′ |
56 | 35 |
| |||
β-actin | 5′-TGGAATCCTGTGGCATCCATGAAAC-3′ 5′-TAAAACGCAGCTCAGTAACAGTCCG-3′ |
61 | 30 |