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. 2016 Jan 14;2016:8981650. doi: 10.1155/2016/8981650

Table 1.

Primer sequences for RT-PCR.

Gene Primer sequence (forward/reverse) T (annealing) Cycles
OPG 5′-TCCTGGCACCTACCTAAAACAGCA-3′ 
5′-CTACACTCTCGGCATTCACTTTGG-3′
57 35

RANKL 5′-ATGATGGAAGGCTCATGGTTG-3′ 
5′-TGTTGGCGTACAGGTAATAGAA-3′
60 35

M-CSF 5′-AAGCAGTAACTGAGCAACGGG-3′ 
5′-CCCATATTGCGACACCGAA-3′
59 30

IL-6 5′-GAAATGAGAAAAGAGTTGTGC-3′ 
5′-ATTGGAAATTGGGGTAGGAAG-3′
56 35

β-actin 5′-TGGAATCCTGTGGCATCCATGAAAC-3′ 
5′-TAAAACGCAGCTCAGTAACAGTCCG-3′
61 30