Table 1.
Bacterial strains and plasmids used in this study
| Strain | Relevant genotype, phenotype | Reference/Source |
|---|---|---|
| LS5494 | Escherichia albertii Albert 19982T | Manan Sharma |
| LS5504 | LS5494(pSIM6), AmpR (Ts) | This study |
| LS5568 | LS5494∆ler::cat, CmR Clone #1 | This study |
| LS5570 | LS5494∆ler::cat, CmR Clone #2 | This study |
| LS5592 | LS5568(pFT-K), CmR KanR (Ts) | This study |
| LS5593 | LS5570(pFT-K), CmR KanR (Ts) | This study |
| LS5598 | LS5494∆grlRA::kan, KanR | This study |
| LS5607 | LS5494∆ler::FRT = LS5592∆cat ∆pFT-K, CmS KanS | This study |
| LS5609 | LS5494∆ler::FRT = LS5593∆cat ∆pFT-K, CmS KanS | This study |
| LS5612 | LS5494∆hfq::cat, CmR | This study |
| DH5α | supE44 ∆lacU169 (Φ80 lacZ∆M15) hsdR17 recA1 endA1 gryA96 thi-1 relA1 | Bettina Bommarius |
| Plasmids | ||
| pSIM6 | p L -gam-bet-exo genes under the control of CI857 repressor, AmpR (Ts) | Don Court |
| pKD46 | p araBAD -gam-bet-exo under the control of AraC, AmpR (Ts) | Barry Wanner |
| pKD3 | Template plasmid to amplify FRT-cat-FRT amplicon, AmpR CmR | Barry Wanner |
| pKD13 | Template plasmid to amplify FRT-kan-FRT amplicon, AmpR KanR | Barry Wanner |
| pFT-K | flp gene under the control of TetR repressor, KanR (Ts) | Fred Blattner |
Tet – Tetracycline, Cm – chloramphenicol, Amp – ampicillin, Kan – Kanamycin, superscripts “R” and “S” denote resistance and sensitivity respectively