Fig. 6.

ERK5 enables NRF2-mediated antioxidant response in cells performing OXPHOS. 10⁷ Jurkat-TAg cells were transfected with 5 μg of the empty pcDNA vector or shERK5 as described in Fig. 4. A) Transfected cells were placed on glucose or OXPHOS media after 24 h and left for the indicated times before mRNA expression was analyzed by qPCR. B) Cells were prepared as in (A), kept in glucose and treated with 20 mM DCA before mRNA analysis. C) Cells prepared as in (B) but they were treated with different concentrations of H₂O₂ for 1 h before analyzing mRNA. The data represent means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 Student's t-test compared to non-treated cells or to empty vector transfected cells when depicted in the graphic.