Fig. 6.

Inhibition of BAF prevents the establishment of latent HIV + infections.

(Panel a) FACS plots show the protocol for quantification of latently infected cells. GFP-negative cells were sorted (left panel) five days after infections. Following sorting, the GFP-negative population (middle panel) was stimulated with PMA and the percentage of reactivated cells was measured 48 h after reactivation (right panel). (Panel b) Infection efficiency in untreated vs BAFi's treated samples was determined by FACS. Bars represent the percent of GFP positive cells (i.e. productive infections) 5 days after infection. (Panel c) Proviral load from the infected population was measured by Alu-PCR. Bars represent the relative amount of HIV-1 DNA compared to untreated control. (Panel d) GFP-negative population (containing uninfected as well as latently infected cells) was treated with PMA for 48 h. Bars represent the percentage of GFP positive cells (i.e. latently infected cells) for each treatment. Data are presented as mean ± SD, * indicates the level of significance at p < 0.05.