Figure 2.

Screening and characterization of halophilic bacterial isolates for protease activity. (a) Equal number of cells of all halophilic isolates (SS1, SS2, SS3, SS5, and SS8) were spotted on LB agar medium supplemented with 1% skim milk and 1 M NaCl. The plates were incubated at 30°C for 24 hours to visualize the zone of clearance due to protease activity. Effect of NaCl, temperature, and pH on protease activity of halophilic isolates: the specific protease activity (U/mg) of the bacterial isolates Halobacillus trueperi SS1, Shewanella algae SS2, and Halobacillus trueperi SS3 was plotted against the different concentrations of sodium chloride (NaCl) (b), varying pH (c), and temperature (d) as indicated. In each panel, the data represent an average of three independent experiments with standard deviation.